ab16325 APOA1 Antibody

Western blot analysis of extracts from various samples, using APOA1 Antibody. Lane 1: Rat brain lysates; Lane 2: Mouse brain lysates; Lane 3: Mouse muscle lysates; Lane 4: Mouse liver lysates;

Western blot analysis of APOA1 expression in Mouse brain lysate.The lane on the right was treated with blocking peptide.

ab16325 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab16325 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab16325 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

FIGURE 1 Characterization of exosomes (EXOs). (A) Purified exosomes were observed under transmission electron microscopy (TEM) using negative staining. (B) The levels of positive exosomal markers TSG101, CD9, and Flotillin-1 in protein lysates of neural stem cells (NSC)-derived exosome pellets were determined by western blotting. (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting. (D,E) Particle-size distribution of exosomes was determined by NanoSight analysis (NTA) in panel (D) and NanoFCM in panel (E) technologies. Scale bar 200 nm in panel (A).

FIGURE 1 | Characterization of exosomes (EXOs). (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting.

Fig. 3. Characterization of the extracellular vesicles (EVs) isolated from porcine follicular fluid. EVs size distribution (A), concentration (B) and zeta potential (C) were assessed by nanoparticle tracking analysis. Data is shown as the mean ± standard deviation (SD) (n = 6). Expression of CD63, β-actin and apolipoprotein A1 (Apo-A1) proteins in EVs samples and granulosa cell lysates (Gc) was demonstrated by Western blot (D). Immunofluorescence was used to confirm CD63 localization in porcine ovarian follicle (E). Positive signal (red) is marked by white arrows. Gc - granulosa cells; Ti - theca interna cells; Te -theca externa cells. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)