ab14375 API-5 Antibody
品牌 |
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产品货号 |
ab14375 |
来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
API-5 |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:1000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
Immunohistochemistry
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.Description
Rabbit polyclonal antibody to API-5
Applications
WB, IF, ICC, IHC.
Immunogen
API-5 Antibody detects endogenous levels of total API-5.
Reactivity
Human, Mouse, Rat.
可预测：Pig(89%), Horse(%), Sheep(%), Dog(%)
Molecular weight
58 KD; 59kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
API-5
Synonyms
AAC 11; AAC-11; AAC11; Antiapoptosis clone 11 protein; API 5; API-5; api5; API5 like 1; API5_HUMAN; API5L1; Apoptosis inhibitor 5; Cell migration inducing gene 8 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; Fibroblast growth factor 2-interacting factor; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
Q9BZZ5
产品图片
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Western blot analysis of extracts from various samples, using API-5 Antibody. Lane 1: Mouse brain, blocked with antigen-specific peptides. Lane 2: Mouse brain. Lane 3: Hepg2 cells(heat-shock treatment). Lane 4: Hela cells(heat-shock treatment). |
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ab14375 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14375) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue). |
