ab17705 APBB1IP Antibody

Western blot analysis of APBB1IP using COLO205 whole lysates.
ab17705 at 1/100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
ab17705 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab17705 1:200) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

品牌

产品货号

ab17705

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

APBB1IP

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用


Western blotting

Recommended dilution: 1:1000-3000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200




最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.

Description
Rabbit polyclonal antibody to APBB1IP

Applications 
WB, IF, ICC, IHC.

Immunogen 
APBB1IP Antibody detects endogenous levels of total APBB1IP.

Reactivity 
Human, Mouse.

Molecular weight
100 kd; 73kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
APBB1IP

Synonyms 
AB1IP; Amyloid beta A4 precursor protein binding family B member 1 interacting protein; Amyloid beta precursor protein binding family B member 1; APBB1 interacting protein 1; INAG1; OTTHUMP00000019344; PREL 1; PREL1; Proline rich EVH1 ligand 1; Proline rich protein 73; Rap1 GTP interacting adapter molecule; RAP1 interacting adaptor molecule; RARP 1; RARP1; Retinoic acid responsive proline rich protein 1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q7Z5R6

产品图片

Western blot analysis of APBB1IP using COLO205 whole lysates.

ab17705 at 1/100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.

ab17705 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab17705 1:200) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

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