ab14627 APBA3 Antibody

Western blot analysis of extracts from Rat liver, using APBA3 Antibody. The lane on the left was treated with blocking peptide.
ab14627 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14627) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).

品牌

产品货号

ab14627

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

APBA3

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用


Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

May modulate processing of the amyloid-beta precursor protein (APP) and hence formation of APP-beta. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.

Description
Rabbit polyclonal antibody to APBA3

Applications 
WB, IF, ICC.

Immunogen 
APBA3 Antibody detects endogenous levels of total APBA3.

Reactivity 
Human, Rat.

Molecular weight
61 KD; 61kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
APBA3

Synonyms 
Adapter protein X11gamma; amyloid beta (A4) precursor protein binding, family A, member 3; Amyloid beta A4 precursor protein-binding family A member 3; Amyloid beta precursor protein binding, family A, member 3 phosphotyrosine binding/ interacting domain (PTB) bearing protein; APBA3; APBA3_HUMAN; lin 10; MGC:15815; Mint 3; Mint-3; Neuron specific X11L2 protein; Neuron-specific X11L2 protein; Neuronal Munc18 1 interacting protein 3; Neuronal Munc18-1-interacting protein 3; phosphotyrosine binding/interacting domain (PTB) bearing protein; X11 like 2 protein 2; X11gamma; X11L2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
O96018

产品图片

Western blot analysis of extracts from Rat liver, using APBA3 Antibody. The lane on the left was treated with blocking peptide.

ab14627 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14627) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).

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