ab17736 AP2S1 Antibody

Western blotting

Recommended dilution: 1:1000-3000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). May also play a role in extracellular calcium homeostasis.

Description
Rabbit polyclonal antibody to AP2S1

Applications 
WB, IF, ICC, IHC.

Immunogen 
AP2S1 Antibody detects endogenous levels of total AP2S1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
17 kDa; 17kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AOX1

Synonyms 
Adapter-related protein complex 2 sigma subunit; Adaptor protein complex AP 2 subunit sigma; Adaptor protein complex AP-2 subunit sigma; Adaptor protein complex AP2 subunit sigma; Adaptor related protein complex 2 sigma 1 subunit; AI043088; AP 17; AP 2 complex subunit sigma 1; AP-2 complex subunit sigma; AP17; AP17 delta; AP2 complex subunit sigma 1; Ap2s1; AP2S1_HUMAN; CLAPS 2; CLAPS2; Clathrin adaptor complex AP2, sigma subunit; Clathrin adaptor protein AP17; Clathrin assembly protein 2 small chain; Clathrin associated/assembly/adaptor protein small 2; Clathrin associated/assembly/adaptor protein small 2, 17-KD; Clathrin associated/assembly/adaptor protein, small 2 (17kD); Clathrin coat assembly protein AP17; Clathrin coat associated protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; MGC62945; Plasma membrane adaptor AP 2 17 kDa protein; Plasma membrane adaptor AP-2 17 kDa protein; Plasma membrane adaptor AP2 17 kDa protein; Sigma adaptin 3b; Sigma2 adaptin; Sigma2-adaptin;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P53680

Western blot analysis of AP2S1 using HepG2 whole lysates. 显示更多 +

ab17736 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. 显示更多 +

ab17736 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab17736 1:200) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue). 显示更多 +