ab13145 AP2B1 Antibody

Western blotting

Recommended dilution: 1:500-1:2000

 

Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.

Description
Rabbit polyclonal antibody to AP2B1

Applications 
WB, IHC.

Immunogen 
AP2B1 Antibody detects endogenous levels of total AP2B1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
105 kDa; 105kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AP2B1 

Synonyms 
Adapter related protein complex 2 beta 1 subunit; Adapter-related protein complex 2 beta subunit; Adaptin, beta 2 (beta); Adaptor protein complex AP-2 subunit beta; Adaptor related protein complex 2, beta 1 subunit; ADTB2; AP-2 complex subunit beta; AP105B; AP2 BETA; Ap2b1; AP2B1_HUMAN; Beta adaptin; Beta-2-adaptin; Beta-adaptin; Beta2 adaptin; CLAPB1; Clathrin assembly protein complex 2 beta large chain; Clathrin associated/assembly/adaptor protein, large, beta 1; DKFZp781K0743; Plasma membrane adaptor HA2/AP2 adaptin beta subunit;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P63010

Western blot analysis of extracts from Rat brain, using AP2B1 Antibody. The lane on the left was treated with blocking peptide. 显示更多 +

Western blot analysis of extracts from K-562 cells, using AP2B1 Antibody. The lane on the left was treated with blocking peptide. 显示更多 +

ab13145 at 1/100 staining mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody. 显示更多 +