ab12135 ANKRD20 Antibody
品牌 |
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产品货号 |
ab12135 |
来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
ANKRD20 |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:1000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
Immunohistochemistry
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Description
Rabbit polyclonal antibody to ANKRD20
Applications
WB, IF, ICC, IHC.
Immunogen
ANKRD20 Antibody detects endogenous levels of total ANKRD20.
Reactivity
Human, Mouse, Rat.
Molecular weight
95kDa; 94kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
ANKRD20
Synonyms
A20A3_HUMAN; ANKRD20A3; Ankyrin repeat domain 20 family member A3; Ankyrin repeat domain 20A related; Ankyrin repeat domain containing protein 20A3; Ankyrin repeat domain-containing protein 20A3; MGC176486; MGC198508;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
Q5VUR7
产品图片
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Western blot analysis of extracts from Hela, using ANKRD20 Antibody. The lane on the left was treated with blocking peptide. |
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ab12135 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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ab12135 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab12135) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue). |
