ab14637 ANGPTL7 Antibody

Western blot analysis of extracts from HepG2 cells using ANGPTL7 antibody.
Western blot analysis of extracts from HepG2, using ANGPTL7 Antibody. The lane on the left was treated with blocking peptide.
Western blot analysis of extracts from various samples, using ANGPTL7 Antibody. Lane 1: Hela cells(H2O2 treatment), blocked with antigen-specific peptides, Lane 2: Hela cells(H2O2 treatment), Lane 3: 293 cells.
ab14637 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14637) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

品牌

产品货号

ab14637

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

ANGPTL7

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用


Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.

Description
Rabbit polyclonal antibody to ANGPTL7

Applications 
WB, IF, ICC.

Immunogen 
ANGPTL7 Antibody detects endogenous levels of total ANGPTL7.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
50 KD; 40kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ANGPTL7

Synonyms 
Angiopoietin like 7; Angiopoietin like factor (CDT6); Angiopoietin like factor; Angiopoietin related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Angiopoietin-related protein 7; ANGL7_HUMAN; ANGPTL7; AngX; CDT6; Cornea derived transcript 6 protein; Cornea-derived transcript 6 protein; dJ647M16.1; OTTHUMP00000001986; RP4-647M16.2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
O43827

产品图片

Western blot analysis of extracts from HepG2 cells using ANGPTL7 antibody.

Western blot analysis of extracts from HepG2, using ANGPTL7 Antibody. The lane on the left was treated with blocking peptide.

Western blot analysis of extracts from various samples, using ANGPTL7 Antibody. Lane 1: Hela cells(H2O2 treatment), blocked with antigen-specific peptides, Lane 2: Hela cells(H2O2 treatment), Lane 3: 293 cells.

ab14637 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14637) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

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