ab15587 ANAPC5 Antibody

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Description
Rabbit polyclonal antibody to ANAPC5

Applications 
WB, IF, ICC, IHC.

Immunogen 
ANAPC5 Antibody detects endogenous levels of total ANAPC5.

Reactivity 
Human, Mouse, Rat.
可预测：Mouse(%), Pig(%), Bovine(%), Horse(%), Sheep(%)

Molecular weight
85 KD; 85kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ANAPC5

Synonyms 
2510006G12Rik; AA408751; AA536819; AA986414; ANAPC 5; ANAPC5; Anaphase promoting complex subunit 5; Anaphase-promoting complex subunit 5; Apc 5; APC5; APC5_HUMAN; Cyclosome subunit 5; PDL 108; PDL108; Periodontal ligament fibroblast protein; RMC1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9UJX4

Western blot analysis of extracts from Mouse testis, using ANAPC5 Antibody at 1/1000 dilution. Observed bands:70kD. 显示更多 +

ab15587 at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody. 显示更多 +