ab14625 AMPD2 Antibody

Western blot analysis of extracts from 293 cells, using AMPD2 Antibody. The lane on the left was treated with blocking peptide.
ab14625 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14625) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

品牌

产品货号

ab14625

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

AMPD2

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用


Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.

Description
Rabbit polyclonal antibody to AMPD2

Applications 
WB, IF, ICC.

Immunogen 
AMPD2 Antibody detects endogenous levels of total AMPD2.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
100 KD; 101kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AMPD2

Synonyms 
Adenosine monophosphate deaminase 2 (isoform L); Adenosine monophosphate deaminase 2; adenosine monophosphate deaminase 2 isoform L; AMP deaminase 2; AMP deaminase isoform L; AMPD 2; AMPD; AMPD2; AMPD2_HUMAN; RP5-1160K1.5; SPG63;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q01433

产品图片

Western blot analysis of extracts from 293 cells, using AMPD2 Antibody. The lane on the left was treated with blocking peptide.

ab14625 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14625) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

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