ab16526 alpha 2 Macroglobulin/A2M Antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
alpha 2 Macroglobulin/A2M |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:2000
Immunohistochemistry
Recommended dilution: 1:50-1:200最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.Description
Rabbit polyclonal antibody to alpha 2 Macroglobulin/A2M
Applications
WB, IHC.
Immunogen
alpha 2 Macroglobulin/A2M Antibody detects endogenous levels of total alpha 2 Macroglobulin/A2M.
Reactivity
Human, Mouse.
Molecular weight
163kDa; 163kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
alpha 2 Macroglobulin/A2M
Synonyms
A2m; A2MG_HUMAN; Alpha 2 M; Alpha 2M; Alpha-2-M; Alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; DKFZp779B086; FWP007; S863 7;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
P01023
产品图片
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Figure 5. Comparison of the re-probed ability of PVDF membrane and NC membrane. Te pooled sera proteins (3.0, 1.5, 0.7, 0.3 and 0.1 μg) were separated by 8% SDS-PAGE, and transferred to PVDF membranes (up) and NC membrane (down), respectively. (a) Staining with AAL and then re-probed with PHA-E; (b) staining with ApoA1 and then re-probing with IgG; (c) Staining with PHA-E and then re-probing with A2M; (d) staining with A2M and then re-probing with PHA-E. Band intensities were statistically analyzed (n=3 individual experiments) and compared using Image Lab sofware (Bio-Rad Laboratories) and GraphPad Prism version 6. Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed *Signifcantly diferent p<0.05, **p<0.01. N.S., not signifcant. All values are means±S.E. (error bars). |
