ab16682 ALDH1A1 Antibody

Western blot analysis of extracts from A549 cells(UV treatment), using ALDH1A1 Antibody. The lane on the left was treated with blocking peptide.
Western blot analysis of extracts from A549 cells(UV treatment), using ALDH1A1 Antibody. The lane on the left was treated with blocking peptide.
Figure 2 Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells. (A) Morphology of cells observed under an inverted light microscope (scale bar, 100 µm). (B) The ultrastructure of cells observed under a transmission electron microscope (x10,000 magnification). (C) Cell sphere-forming rate evaluated using the sphere formation assay (x100 magnification). (D) Number of spheres measured using the colony-formation assay (x200 magnification). (E) Expression levels of CD44, ALDH1, Nanog and Snail determined using western blotting assays. *P
Figure 7 Growth of stem cells and EMT progression in SW1990/GZ xenograft model is inhibited by Emo by inactivating Snail. (A) Expression levels of CD44, ALDH1, Nanog and Snail as determined via western blotting. (B) Expression level of CD44, ALDH1, Nanog and Snail in tumor tissues measured via immunohistochemistry (x200 magnification). (C) Expression levels of vimentin and E-cadherin as determined via western blotting. (D) Expression levels of vimentin and E-cadherin in tumor tissues measured via immunohistochemistry (x200 magnification). *P

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

ALDH1A1

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:2000

 

Immunohistochemistry

Recommended dilution: 1:50-1:200




最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo.

Description
Rabbit polyclonal antibody to ALDH1A1

Applications 
WB, IHC.

Immunogen 
ALDH1A1 Antibody detects endogenous levels of total ALDH1A1.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(91%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)

Molecular weight
55kDa; 55kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ALDH1A1

Synonyms 
Acetaldehyde dehydrogenase 1; AHD2; AL1A1_HUMAN; ALDC; Aldehyde dehydrogenase 1 family member A1; Aldehyde dehydrogenase 1 soluble; Aldehyde dehydrogenase 1A1; Aldehyde dehydrogenase; Aldehyde dehydrogenase cytosolic; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase liver cytosolic; ALDH 1; ALDH 1A1; ALDH class 1; ALDH, liver cytosolic; ALDH-E1; ALDH1 A1; ALDH1; ALDH11; ALDH1A1; ALHDII; cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; HEL-S-53e; MGC2318; PUMB1; RALDH 1; RalDH1; Retinal dehydrogenase 1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P00352

产品图片

Western blot analysis of extracts from A549 cells(UV treatment), using ALDH1A1 Antibody. The lane on the left was treated with blocking peptide.

Western blot analysis of extracts from A549 cells(UV treatment), using ALDH1A1 Antibody. The lane on the left was treated with blocking peptide.

Figure 2 Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells. (A) Morphology of cells observed under an inverted light microscope (scale bar, 100 µm). (B) The ultrastructure of cells observed under a transmission electron microscope (x10,000 magnification). (C) Cell sphere-forming rate evaluated using the sphere formation assay (x100 magnification). (D) Number of spheres measured using the colony-formation assay (x200 magnification). (E) Expression levels of CD44, ALDH1, Nanog and Snail determined using western blotting assays. *P

Figure 7 Growth of stem cells and EMT progression in SW1990/GZ xenograft model is inhibited by Emo by inactivating Snail. (A) Expression levels of CD44, ALDH1, Nanog and Snail as determined via western blotting. (B) Expression level of CD44, ALDH1, Nanog and Snail in tumor tissues measured via immunohistochemistry (x200 magnification). (C) Expression levels of vimentin and E-cadherin as determined via western blotting. (D) Expression levels of vimentin and E-cadherin in tumor tissues measured via immunohistochemistry (x200 magnification). *P

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