ab13995 AKAP5 Antibody

Western blot analysis of extracts from mouse lung, using AKAP5 Antibody. Lane 1 was treated with the blocking peptide.
ab13995 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

AKAP5

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.

Description
Rabbit polyclonal antibody to AKAP5

Applications 
WB, IF, ICC.

Immunogen 
AKAP5 Antibody detects endogenous levels of total AKAP5.

Reactivity 
Human, Mouse.
可预测:Pig(82%), Horse(%), Dog(%)

Molecular weight
47 KD; 47kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AKAP5

Synonyms 
A kinase (PRKA) anchor protein 5; A kinase anchor protein 5; A kinase anchor protein 79kDa; A kinase anchoring protein 75/79; A-kinase anchor protein 5; A-kinase anchor protein 79 kDa; AKAP 5; AKAP 75; AKAP 79; AKAP-5; Akap5; AKAP5_HUMAN; AKAP75; AKAP79; cAMP dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; H21;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P24588

产品图片

Western blot analysis of extracts from mouse lung, using AKAP5 Antibody. Lane 1 was treated with the blocking peptide.

ab13995 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

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