ab16838 AK4 Antibody

Western blot analysis of extracts from Rat kidney, using AK4 Antibody. The lane on the left was treated with blocking peptide.
ab16838 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab16838 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

AK4

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:2000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity. Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK. Plays a protective role in the cellular response to oxidative stress.

Description
Rabbit polyclonal antibody to AK4

Applications 
WB, IF, ICC, IHC.

Immunogen 
AK4 Antibody detects endogenous levels of total AK4.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
25kDa; 25kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AK4

Synonyms 
Ah receptor; AhR; AHR_HUMAN; Aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; Aryl hydrocarbon receptor precursor; bHLHe76; Class E basic helix loop helix protein 76; Class E basic helix-loop-helix protein 76; HGNC:348;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P27144

产品图片

Western blot analysis of extracts from Rat kidney, using AK4 Antibody. The lane on the left was treated with blocking peptide.

ab16838 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab16838 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

联系经销商
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