ab14374 AIM2 Antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
AIM2 |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:1000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.Description
Rabbit polyclonal antibody to AIM2
Applications
WB, IF, ICC.
Immunogen
AIM2 Antibody detects endogenous levels of total AIM2.
Reactivity
Human, Mouse.
可预测：Horse(88%)
Molecular weight
39 KD; 39kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
AIM2
Synonyms
Absent in melanoma 2; AIM 2; Aim2; AIM2_HUMAN; Interferon-inducible protein AIM2; OTTHUMP00000035296; PYHIN4;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
O14862
产品图片
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Western blot analysis of extracts from mouse brain, using AIM2 Antibody. Lane 1 was treated with the antigen-specific peptide. |
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Western blot analysis of extracts from Mouse brain, using AIM2 Antibody. The lane on the left was treated with blocking peptide. |
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Figure 4 Effect of AICAR treatment on NLRP3, NLRP1, NLRC4, and AIM2 inflammasome components (A,C) Representative immunofluorescence staining images showing the expressions of NLRP3 and caspase-1 in the spinal dorsal horn of sham and CIBP rats. Scale bar: 20 μm. (B,D) Quantitative analysis of fluorescence intensity in (A) and (C). Data are expressed as the mean±SD ( n=3). * P |
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Fig. 5. Effects of Rg1 treatment on inflammation in LPS-induced CKD mice. (A) Representative WB images of AIM2, Caspase-1 p20, IL-6, and Cleaved-IL-1β. (B) Statistical results of AIM2. (C) Statistical results of Caspase-1 p20. (D) Statistical results of IL-6. (E) Statistical results of Cleaved-IL-1β. (F) Statistical results of Il1b and Casp1 mRNA (qPCR). (G) Representative images of AIM2 (IF, ×400, bar: 20 μm). (H) Statistical results of AIM2 IF. Data are expressed as mean ± SD, WB and IF, n = 4, qPCR, n = 3. **P < 0.01 versus Control; #P < 0.05, ##P < 0.01 versus Model. |
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Fig. 5. Effects of Rg1 treatment on inflammation in LPS-induced CKD mice. (A) Representative WB images of AIM2, Caspase-1 p20, IL-6, and Cleaved-IL-1β. (B) Statistical results of AIM2. (C) Statistical results of Caspase-1 p20. (D) Statistical results of IL-6. (E) Statistical results of Cleaved-IL-1β. (F) Statistical results of Il1b and Casp1 mRNA (qPCR). (G) Representative images of AIM2 (IF, ×400, bar: 20 μm). (H) Statistical results of AIM2 IF. Data are expressed as mean ± SD, WB and IF, n = 4, qPCR, n = 3. **P < 0.01 versus Control; #P < 0.05, ##P < 0.01 versus Model. |
