ab11476 AhR Antibody

Western blot analysis of extracts from various samples, using AhR Antibody. Lane 1: Mouse heart, blocked with antigen-specific peptides. Lane 2: Mouse heart. Lane 3: Pc12 cells.

ab11476 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab11476 at 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab11476 at 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab11476 at 1/100 staining Rat ovary tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

FIGURE 6 | Compared with that in the control group, decreased IL-10 and TGF-β expression in colon tissues of mice was induced by DSS and detected by immunohistochemistry.Representative photographs of immunohistochemical staining of IL-10 and TGF-β at the indicated time point in each group (A). Scale bars = 50 µm. (B) The mRNA level of IL-10 and TGF-β in colon tissues of different groups. (C) Protein expression of AhR,IL-10, and TGF-β in colon tissues of different groups.

FIGURE 5 | Compared with that in the control group, decreased AHR expression in colon tissues of mice was induced by DSS and detected by immunohistochemistry (A), Representative colon images at the indicated time point in each group. Scale bars = 50 µm. Representative example of an immunofluorescence double staining of Foxp3 (Red) and AhR (Green) in colon tissues (B).

FIGURE 5 | Compared with that in the control group, decreased AHR expression in colon tissues of mice was induced by DSS and detected by immunohistochemistry (A), Representative colon images at the indicated time point in each group. Scale bars = 50 µm. Representative example of an immunofluorescence double staining of Foxp3 (Red) and AhR (Green) in colon tissues (B).

Fig. 7. |Hydrogen inhibited pulmonary AhR protein decline in the lung tissues of rats when exposed to PM2.5. Pulmonary AhR protein expression was determined by western blotting (A). AhR band relative intensities were quantified and normalized to GAPDH (B).

FIGURE 7 Effects of emodin and AhR inhibitor CH223191 on the protein expression levels of AhR and CYP1A1 in MCF-7 cells (mean ± SD, n = 3) (A) In the cells treated with emodin, the expression of AhR protein using GAPDH as a loading control and the graphical representations of the AhR/GAPDH ratio; (B) In the cells treated with emodin, the expression of CYP1A1 protein using GAPDH as a loading control and the graphical representations of the CYP1A1/GAPDH ratio; (C) In the cells treated with CH223191, the expression of CYP1A1 protein using GAPDH as a loading control and the graphical representations of the CYP1A1/GAPDH ratio; (D) In the cells intervened with emodin and 10 μmol/L of CH223191, the expression of CYP1A1 protein using GAPDH as a loading control and the graphical representations of the CYP1A1/GAPDH ratio.