ab14609 AGXT2L2 Antibody

Western blot analysis of extracts from HepG2 cells using AGXT2L2 antibody.
Western blot analysis of extracts from HepG2, using AGXT2L2 Antibody. The lane on the left was treated with blocking peptide.
ab14609 at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
ab14609 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14609) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

AGXT2L2

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200




最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Catalyzes the pyridoxal-phosphate-dependent breakdown of 5-phosphohydroxy-L-lysine, converting it to ammonia, inorganic phosphate and 2-aminoadipate semialdehyde.

Description
Rabbit polyclonal antibody to AGXT2L2

Applications 
WB, IF, ICC, IHC.

Immunogen 
AGXT2L2 Antibody detects endogenous levels of total AGXT2L2.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(91%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)

Molecular weight
50 KD; 50kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AGXT2L2

Synonyms 
2900006B13Rik; 5-phosphohydroxy-L-lysine phospho-lyase; Alanine glyoxylate aminotransferase 2 like 2; MGC117348; MGC15875; MGC45484; OTTMUSP00000005767; PHLU; PP9286; RP23-79E13.3;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q8IUZ5

产品图片

Western blot analysis of extracts from HepG2 cells using AGXT2L2 antibody.

Western blot analysis of extracts from HepG2, using AGXT2L2 Antibody. The lane on the left was treated with blocking peptide.

ab14609 at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.

ab14609 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14609) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

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