ab15708 AGTRL1 Antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
AGTRL1 |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:1000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
Immunohistochemistry
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity. Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone (By similarity). May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis (By similarity). Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development (By similarity). Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure.
(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
Description
Rabbit polyclonal antibody to AGTRL1
Applications
WB, IF, ICC, IHC.
Immunogen
AGTRL1 Antibody detects endogenous levels of total AGTRL1.
Reactivity
Human, Mouse, Rat.
可预测：Bovine(83%), Horse(%), Rabbit(%), Dog(%)
Molecular weight
43 KD; 43kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
AGTRL1
Synonyms
AGTR L1; AGTRL 1; AGTRL1; Angiotensin II receptor like 1; Angiotensin receptor like 1; Angiotensin receptor-like 1; Apelin receptor; APJ (apelin) receptor; APJ; APJ receptor; APJ_HUMAN; APJR; APLNR; B78; FLJ90771; G protein coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; GPCR34; HG 11; HG11; HG11 orphan receptor; MGC45246; MSR; msr/apj;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
P35414
产品图片
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Western blot analysis of extracts from EC304 cells(heat-shock treatment), using AGTRL1 Antibody at 1/1000 dilution. Observed bands:50kD. |
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ab15708 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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ab15708 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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ab15708 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#ab15708) and mouse anti-beta tubulin Ab(#T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue). |
