ab14499 AGPAT4 Antibody

Western blot analysis of extracts from Hepg2, using AGPAT4 Antibody. Lane 1 was treated with the blocking peptide.
ab14499 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

AGPAT4

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Exhibits high acyl-CoA specificity for polyunsaturated fatty acyl-CoA, especially docosahexaenoyl-CoA (22:6-CoA, DHA-CoA) (By similarity).

Description
Rabbit polyclonal antibody to AGPAT4

Applications 
WB, IF, ICC.

Immunogen 
AGPAT4 Antibody detects endogenous levels of total AGPAT4.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(90%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)

Molecular weight
44 KD; 44kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AGPAT4

Synonyms 
1 acyl sn glycerol 3 phosphate acyltransferase delta; 1 acylglycerol 3 phosphate O acyltransferase 4; 1 AGP acyltransferase 4; 1 AGPAT4; 1-acyl-sn-glycerol-3-phosphate acyltransferase delta; 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta); 1-acylglycerol-3-phosphate O-acyltransferase 4; 1-AGP acyltransferase 4; 1-AGPAT 4; AGPAT4; dJ473J16.2; LPAAT delta; LPAAT-delta; Lysophosphatidic acid acyltransferase delta; Lysophosphatidic acid acyltransferase-delta (LPAAT-delta); PLCD_HUMAN; RP3 473J16.2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9NRZ5

产品图片

Western blot analysis of extracts from Hepg2, using AGPAT4 Antibody. Lane 1 was treated with the blocking peptide.

ab14499 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

联系经销商
北京晶莱华科生物技术有限公司
北京总部电话:4008-766-085
邮箱:beijing@genlay.cn
地址:北京经济技术开发区科创十三街12号院德为科技园1号楼5层
上海分公司电话:4008-766-085
邮箱:shanghai@genlay.cn
地址:上海市青浦区普仓路648弄71号
长沙分公司电话:4008-766-085
邮箱:changsha@genlay.cn
地址:长沙市岳麓区麓谷大道627号新长海中心A1栋1203室
http://www.genlay.cn
http://www.genecro.cn
genetex@neobioscience.com