ab12867 AGO2 Antibody

Western blotting

Recommended dilution: 1:500-1:2000

 

Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Description
Rabbit polyclonal antibody to AGO2

Applications 
WB, IHC.

Immunogen 
AGO2 Antibody detects endogenous levels of total AGO2.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Xenopus(%)

Molecular weight
90-95 kDa; 97kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AGO2

Synonyms 
Ago 2; AGO2_HUMAN; Argonaute 2; argonaute 2, RISC catalytic component; Argonaute RISC catalytic component 2; Argonaute2; CTA-204B4.6; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; Eukaryotic translation initiation factor 2C subunit 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9UKV8

Western blot analysis of extracts from K-562 cells, using AGO2 antibody. 显示更多 +

ab12867 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody. 显示更多 +

Figure 6 Confocal microscopic analysis of AGO2 expression in OS cells. Representative immunofluorescence analysis results for regulation of AGO2 by HuR and lncRNA XIST. SJSA-1 cells with different treatments were fixed with 1% paraformaldehyde and stained with anti-HuR (red) and anti-AGO2 (green) antibodies. Nuclei of cells appeared as blue because of DAPI nuclear staining. (A) Cells were transfected with an NC siRNA for 48 h. (B) Cells were transfected with a HuR siRNA for 48 h. (C) Cells were transfected with an lncRNA XIST siRNA for 48 h. (D) Cells were co-transfected with both HuR and lncRNA XIST siRNAs for 48 h. Stained cells were examined under a Leica confocal immunofluorescence microscope. Upper panel: original magnification 200x; lower panel, original magnification 630x. 显示更多 +