ab12730 AGGF1 Antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
AGGF1 |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:2000
Immunohistochemistry
Recommended dilution: 1:50-1:200最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Promotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion.Description
Rabbit polyclonal antibody to AGGF1
Applications
WB, IHC.
Immunogen
AGGF1 Antibody detects endogenous levels of total AGGF1.
Reactivity
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Dog(%), Chicken(%), Xenopus(%)
Molecular weight
84-100 kDa; 81kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
AGGF1
Synonyms
AGGF 1; Aggf1; AGGF1_HUMAN; Angiogenic factor VG5Q; Angiogenic factor with G patch and FHA domains 1; G patch domain containing protein 7; G patch domain-containing protein 7; GPATC 7; GPATC7; GPATCH 7; GPATCH7; HSU84971; HUS84971; hVG5Q; Vasculogenesis gene on 5q; Vasculogenesis gene on 5q protein; VG5Q;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
Q8N302
产品图片
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Western blot analysis of extracts from 293, using AGGF1 antibody. Lane 1 was treated with the blocking peptide. |
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ab12730 at 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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ab12730 at 1/100 staining Human normal tissues adjacent to liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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ab12730 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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Figure 1. |Immunostaining for AGGF1 (angiogenic factor with G-patch and FHA domain 1), FOXC2 (forkhead box C2), and E-cad (E-cadherin) in esophageal squamous cell carcinoma and control tissue. A, Positive AGGF1 in the cytoplasm of esophageal squamous cell carcinoma tissue (40 magnification) |
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Figure 1 Immunostaining of UBE2C, AGGF1, VM and MVD in NSCLC and control tissues. (A) Positive staining of UBE2C in NSCLC tissues (×400). (B) Negative staining of UBE2C in control tissues (×400). (C) Positive staining of AGGF1 in NSCLC tissues (×400). (D) Negative staining of AGGF1 in control tissues (×400). (E) Positive staining of VM in NSCLC tissues (×400). (F) Negative staining of VM in control tissues (×400). (G) High MVD in NSCLC tissues (×400). (H) Low MVD in control tissues (×400). |
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Figure 1 The expression of the three proteins in SOC and ovarian serous cystadenoma (ElivisionTM, ×400; (A) NCAPH in SOC; proportion of positive cells; (B) NCAPH in ovarian serous cystadenoma; (C) AGGF1 in SOC; (D) AGGF1 in ovarian serous cystadenoma; (E) FOXC2 in SOC; (F) FOXC2 in ovarian serous cystadenoma). |
