ab16463 AGER/RAGE Antibody

Western blotting

Recommended dilution: 1:500-1:2000
 

Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.

Description
Rabbit polyclonal antibody to AGER/RAGE

Applications 
WB, IHC.

Immunogen 
AGER/RAGE Antibody detects endogenous levels of total AGER/RAGE.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(88%), Horse(%), Rabbit(%)

Molecular weight
43~60kDa; 43kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AGER/RAGE

Synonyms 
Advanced glycosylation end product-specific receptor; Ager; MGC2235; RAGE_HUMAN; Receptor for advanced glycosylation end products;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q15109

Fig. 5. Effect of crocin on hippocampal HMGB-1 level and expression as well as RAGE expression in a rat model of CS-induced cognitive disorders. (A) HMGB1 level (pg/g tissue); n = 6, (B) western blot showing HMGB1 and RAGE expression, (C) bar graph reflecting HMGB1 expression, and (D) bar graph reflecting RAGE expression. Protein expression level was normalized to β-actin and shown relative to that of control; n = 3. Values were expressed as means ± SD. Significant difference were presented at *p < 0.05 and ***p < 0.001; using One-way ANOVA followed by Tukey 显示更多 +