ab10927 AFP Antibody

Western blot analysis of extracts from Rat liver, using AFP Antibody. The lane on the left was treated with blocking peptide.

ab10927 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab10927 at 1/100 staining human breast tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Figure 5. Analysis of hepatic protein markers after 3 weeks of hepatic differentiation. (A) H&E staining of hepatic differentiation of mesenchymal stem cells (MSCs) in all groups. Immunofluorescence analysis of (B) alpha fetoprotein (AFP), (C) cytokeratin 19 (CK19) and (D) albumin (ALB) expression in all groups. Undifferentiated MSCs were used as controls. Scale bar, 100 µm. 2D, 2-dimensional; 3D, 3-dimensional; DLS, decellularized liver scaffold.

FIGURE 1 | Irradiation-induced liver damage in mice. C57BL/6J mice were exposed to 8.0 Gy of total body irradiation (TBI). Liver tissues were harvested at days 1, 3, and 7 post-exposure (n = 6). (A) Representative pictures by HE staining are shown. Scale bar = 50 μm. Arrows indicate edema hepatocytes. (B and C) ALB and AFP expression in liver tissues (n = 3). Expression of ALB (B) and AFP (C) was detected by western blotting.

Figure 6 GA suppresses tumor growth and enhances the antitumor effect of sorafenib. Images of tumors (A), tumor volumes (B), and body weight (C) of PLC/PRF/5 transfected with shNC or shJNK1 in BALB/c nu/nu mice treated with 100 mg/kg GA or saline as control. (D) IHC staining indicates the expression of CSC markers (SOX2 and OCT4) and differentiation markers (AFP and HEPPAR1) in tumors. (E) IHC analysis of SOX2, OCT4, AFP, and HEPPAR1 in tumors (one-way ANOVA; ***P < 0.001). Scale bars in (D), 30 μm. Images of tumors (F), tumor volumes (G) and body weight (H) of PLC/PRF/5-bearing BALB/c nu/nu mice. GA or sorafenib treatment obviously inhibited tumor growth. Furthermore, GA could enhance the anti-tumor effect of sorafenib.

Fig. 7 The effects of hUC-MSC transplantation on hepatocyte regeneration in ACLF rats. Liver sections from ACLF rats 12 and 24 h post-hUC-MSC transplantation or 0.9% sodium chloride injection as a control were used for immunohistochemical staining of AFP, CK18, HGF, and PCNA and microscopic examination (n = 4/group). Representative photographs are shown (a). Positive staining was quantified and is presented as the mean ± SD (b, c). d Serum levels of HGF were detected by ELISA (4h, n = 3/group; 12h and 24h, n = 4/group). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Figure 4. Analysis of hepatic protein markers in mouse MSCs following 23 days of hepatic differentiation. Green immunofluorescence staining of ALB, AFP and CK19 in undifferentiated MSC control, 2D and 3D groups. Nuclei were stained blue with DAPI. Scale bars, 100 µm. MSCs, mesenchymal stem cells; ALB, albumin; AFP, α-fetoprotein; CK19, cytokeratin-19.