ab14081 AF10 Antibody

ab14081 at 1/100 staining human Testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

AF10

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation.

Description
Rabbit polyclonal antibody to AF10

Applications 
WB, IF, ICC, IHC.

Immunogen 
AF10 Antibody detects endogenous levels of total AF10.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)

Molecular weight
109 KD; 113kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AF10

Synonyms 
AF 10; AF10; AF10_HUMAN; ALL 1 fused gene from chromosome 10; ALL1 fused gene from chromosome 10; ALL1-fused gene from chromosome 10 protein; DKFZp686E10210; MGC75086; MLLT 10; MLLT10; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10; Myeloid/lymphoid or mixed lineage leukemia translocated to 10; OTTHUMP00000045273; Protein AF 10; Protein AF-10; Protein AF10; Type I AF10 protein;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P55197

产品图片

ab14081 at 1/100 staining human Testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

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