ab13974 Adrenergic Receptor alpha -2C Antibody

Western blot analysis of Adrenergic Receptor α-2C expression in mouse brain lysate
ab13974 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF3108 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

Adrenergic Receptor alpha -2C

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

Description
Rabbit polyclonal antibody to Adrenergic Receptor alpha -2C

Applications 
WB, IF, ICC.

Immunogen 
Adrenergic Receptor alpha -2C Antibody detects endogenous levels of total Adrenergic Receptor alpha -2C.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Horse(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
70 KD; 50kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
Adrenergic Receptor  alpha -2C

Synonyms 
Adra 2c; ADRA2C; ADRA2L2; ADRA2RL2; ADRARL2; Adrenergic alpha 2C receptor; Adrenoceptor alpha 2C; Alpha 2C adrenergic receptor; Alpha 2C adrenergic receptor subtype C4; Alpha 2C adrenoceptor; Alpha 2C adrenoreceptor; Alpha 2CAR; Alpha-2-adrenergic receptor, renal type; alpha2 AR C4; alpha2 C4; alpha2C; ALPHA2CAR; alpha2cii-adrenergic receptor; Subtype alpha2 C4;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P18825

产品图片

Western blot analysis of Adrenergic Receptor α-2C expression in mouse brain lysate

ab13974 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF3108 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

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