ab15705 ADORA2A Antibody

ab15705 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Fig. 7. EA upregulated the expression of A2AR, cAMP, PKA and p-CREB in the spinal dorsal horn after SNL, while SCH58261 had the opposite effects. (A), (C), (D), (E) Representative Western blots and quantification data of the expression of A2AR/GAPDH, PKA/GAPDH and p-CREB/CREB in each group. n = 5. (B) The content of cAMP in the spinal dorsal horn measured by ELISA. n = 6. (F), (G) qPCR showing the expression of A2AR and PKA mRNA in the spinal cord. n = 6. (H) Immunofluorescence of A2AR-, cAMP- and PKA-positive cells in the spinal dorsal horn. Scale bars, 200 μm. (I), (J), (K) Number of A2AR-, cAMP- and PKA-positive cells in the spinal dorsal horn (lamina I-II and lamina III-IV). n = 4. Columns represent the mean ± SD. $$p < 0.01 vs. the S group; ##p < 0.01 vs. the M group; &&p < 0.01 vs. the M + EA group.