ab14083 ADK Antibody

Western blot analysis of extracts from various samples, using ADK Antibody. Lane 1: Hela cells, blocked with antigen-specific peptides, Lane 2: Hela cells, Lane 3: P19 cells.
ab14083 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14083) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB

实验种属

Human,Mouse,Rat,Rabbit,Pig

偶联标记

Unconjugated

目的蛋白

ADK

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

ATPdependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

Description
Rabbit polyclonal antibody to ADK

Applications 
WB, IF, ICC.

Immunogen 
ADK Antibody detects endogenous levels of total ADK.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
40 KD; 41kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ADK

Synonyms 

2310026J05Rik; 5033405D03Rik; Adenosine 5''-phosphotransferase; Adenosine kinase; adk; ADK_HUMAN; AI255373; AI987814; AK; MGC6593; OTTHUMP00000019864; OTTHUMP00000019865;


Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P55263

产品图片

Western blot analysis of extracts from various samples, using ADK Antibody. Lane 1: Hela cells, blocked with antigen-specific peptides, Lane 2: Hela cells, Lane 3: P19 cells.

ab14083 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14083) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).

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