ab11474 ADD1 Antibody

Western blot analysis of extracts from various samples, using ADD1 Antibody. Lane 1: Rat heart treated with blocking peptide; Lane 2: Rat heart; Lane 3: VERO.
ab11474 at 1/100 staining human brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,IP,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

ADD1

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:2000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200


Immunoprecipitation

Recommended dilution: 

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Description
Rabbit polyclonal antibody to ADD1

Applications 
WB, IF, ICC, IHC, IP.

Immunogen 
ADD1 Antibody detects endogenous levels of total ADD1.

Reactivity 
Human, Mouse, Rat, Monkey.
可预测:Zebrafish(100%), Bovine(%), Horse(%), Dog(%), Xenopus(%)

Molecular weight
80kDa; 81kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ADD1

Synonyms 
ADD A; ADD1; ADDA; ADDA_HUMAN; Adducin 1 (alpha); Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427; OTTHUMP00000151163; OTTHUMP00000151164; OTTHUMP00000151165; OTTHUMP00000218664; OTTHUMP00000218665; ADD1; ADD2; ADD3; ADDA; ADDB; ADDL; Adducin 1 (alpha); Adducin 1; Adducin 2 (beta); Adducin 2; Adducin 3 (gamma); Adducin 3; Adducin like; Alpha adducin; Beta adducin;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P35611 | P35612

产品图片

Western blot analysis of extracts from various samples, using ADD1 Antibody. Lane 1: Rat heart treated with blocking peptide; Lane 2: Rat heart; Lane 3: VERO.

ab11474 at 1/100 staining human brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

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