ab14600 ADAMTS19 Antibody

Western blot analysis of extracts from Hela, using ADAMTS19 Antibody.
ab14600 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

ADAMTS19

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200




最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

The precursor is cleaved by a furin endopeptidase.

 

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).

Description
Rabbit polyclonal antibody to ADAMTS19

Applications 
WB, IF, ICC, IHC

Immunogen 
ADAMTS19 Antibody detects endogenous levels of total ADAMTS19.

Reactivity 
Human,Mouse.
可预测:Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Molecular weight
140 KD; 134kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ADAMTS19

Synonyms 
A disintegrin and metalloproteinase with thrombospondin motifs 19; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 19; A disintegrin-like and metalloproteinase with thrombospondin type 1 motif, 19; ADAM metallopeptidase with thrombospondin type 1 motif, 19; ADAM TS 19; ADAM TS19; ADAMTS 19; ADAMTS19; ATS19; EC 3.4.24.-; FLJ16042;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q8TE59
 

产品图片

Western blot analysis of extracts from Hela, using ADAMTS19 Antibody.

ab14600 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

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