ab11086 ADAM10 Antibody

Western blotting

Recommended dilution: 1:500-1:2000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Enhances the cleavage of CHL1 by BACE1 (By similarity). Cleaves NRCAM (By similarity). Cleaves TREM2, resulting in shedding of the TREM2 ectodomain. Involved in the development and maturation of glomerular and coronary vasculature (By similarity). During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions (By similarity). May regulate the EFNA5-EPHA3 signaling.

(Microbial infection) Promotes the cytotoxic activity of S.aureus hly by binding to the toxin at zonula adherens and promoting formation of toxin pores.

Description
Rabbit polyclonal antibody to ADAM10

Applications 
WB, IF, ICC, IHC

Immunogen 
ADAM10 Antibody detects endogenous levels of total ADAM10.

Reactivity 
Human.
可预测：Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(86%)

Molecular weight
70,95kD; 84kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ADAM10

Synonyms 
A disintegrin and metalloprotease domain 10; A disintegrin and metalloproteinase domain 10; AD 10; AD10; AD18; ADA10_HUMAN; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD 156c; CD156c; CD156c antigen; CDw156; disintegrin and metalloproteinase domain containing protein 10; Disintegrin and metalloproteinase domain-containing protein 10; HsT 18717; HsT18717; Kuz; Kuzbanian; Kuzbanian protein homolog; Kuzbanian, Drosophila, homolog of; MADM; Mammalian disintegrin metalloprotease; Mammalian disintegrin-metalloprotease; RAK;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
O14672