ab11550 ADAM 17 Antibody

Western blot analysis of ADAM 17 expression in HT1080 whole cell lysates.

ab11550 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab11550) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

ab11550 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab11550 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

Fig. 6. |ADAM17 suppression enhanced matrix metabolism in NP cells.(A,B) Western blotting (A) and subsequent densitometric analysis (B) revealed that collagen IIa and aggrecan protein expression in rat NP cells was increased by the ADAM17 inhibitor. GAPDH was used as the control. (C) Immunofluorescence imaging of human NP cells treated with LV-shControl and LV-shADAM17. (D, E)Western blotting (D) and subsequent densitometric analysis (E) demonstrated that ADAM17 suppression by LV-shADAM17 markedly increased collagen IIa and aggrecan protein expression in human NP cells.

Fig. 6. Change in macrophage secreted ADAM-17 in vitro and in vivo. Levels of ADAM-17 in BALF (A, n = 5 per group) and expression levels of ADAM-17 in mouse lung tissues (B, n = 3 per group). C Levels of ADAM-17 in cell supernatants from differently polarized macrophages. D Representative image and densitometry of immunofluorescence of ADAM-17 in different polarized macrophages (Scar bar, 20 μm). Relative protein expression E and mRNA F of ADAM-17 in different polarized macrophages. Based on three independent experiments, all data were shown as mean ± SD.

Fig. 6. Change in macrophage secreted ADAM-17 in vitro and in vivo. Levels of ADAM-17 in BALF (A, n = 5 per group) and expression levels of ADAM-17 in mouse lung tissues (B, n = 3 per group). C Levels of ADAM-17 in cell supernatants from differently polarized macrophages. D Representative image and densitometry of immunofluorescence of ADAM-17 in different polarized macrophages (Scar bar, 20 μm). Relative protein expression E and mRNA F of ADAM-17 in different polarized macrophages. Based on three independent experiments, all data were shown as mean ± SD.