ab10581 ACSS1 Antibody

Western blot analysis of extracts from TSA treated samples, using Acetyl-ACSS1 (Lys642) Antibody. Lane1: COLO205 cell lysates, Lane2: Hybridoma cell lysates, Lane3: MCF7 cell lysates, Lane4: 293 cell lysates, Lane5: Myeloma cell lysates, Lane6: K652 cell lysates.
ab10581 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ab10581 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

ACSS1

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:2000
 


Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity (By similarity). Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (By similarity). Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (By similarity).

Description
Rabbit polyclonal antibody to ACSS1

Applications 
WB, IHC.

Immunogen 
ACSS1 Antibody detects endogenous levels of total ACSS1.

Reactivity 
Human, Mouse, Rat.

Molecular weight
70 kDa; 75kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ACSS1

Synonyms 
ACAS2L; AceCS2; AceCS2L; Acetate CoA ligase 2; Acetate--CoA ligase 2; Acetyl CoA synthetase 2; Acetyl Coenzyme A synthetase 2 (AMP forming) like; Acetyl coenzyme A synthetase 2 like, mitochondrial; Acetyl-CoA synthetase 2; Acetyl-coenzyme A synthetase 2-like, mitochondrial; ACS2L_HUMAN; Acss1; Acyl CoA synthetase short chain family member 1; Acyl-CoA synthetase short-chain family member 1; FLJ45659; KIAA1846; MGC33843;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9NUB1

产品图片

Western blot analysis of extracts from TSA treated samples, using Acetyl-ACSS1 (Lys642) Antibody. Lane1: COLO205 cell lysates, Lane2: Hybridoma cell lysates, Lane3: MCF7 cell lysates, Lane4: 293 cell lysates, Lane5: Myeloma cell lysates, Lane6: K652 cell lysates.

ab10581 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab10581 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

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