ab17039 ACK1 Antibody

Western blotting

Recommended dilution: 1:500-1:2000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.

Description
Rabbit polyclonal antibody to ACK1

Applications 
WB, IF, ICC, IHC

Immunogen 
ACK1 Antibody detects endogenous levels of total ACK1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(100%), Rabbit(88%), Dog(100%), Chicken(100%), Xenopus(88%)

Molecular weight
114kDa; 115kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ACK1

Synonyms 
Acetate kinase 1; Acetokinase 1; ACK 1; ACK; ACK-1; ACK1; ACK1_HUMAN; Activated Cdc42 associated kinase 1; Activated CDC42 kinase 1; Activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK 2; TNK2; Tyrosine kinase non receptor 2; Tyrosine kinase non receptor protein 2; Tyrosine kinase non-receptor protein 2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q07912

Western blot analysis of extracts from 3T3-L1P6(heat shock treatment), using ACK1 Antibody. The lane on the left was treated with blocking peptide. 显示更多 +

Figure 2 TNK2 was overexpressed and directly binds to miR-125a-3p in colon cancer cells. (A,B) Relative expression of TNK2 in COAD and normal colon cells, measured by RT-qPCR and western blot. (C,D) Relative expression of TNK2 after transfection of SW620 cells was measured by RT-qPCR. (E) Putative binding sites involving the TNK2 3′UTR and miR-125a-3p were predicted by miRBase. (F) Dual-luciferase reporter assays were performed to test putative binding sites in TNK2 3′UTR and miR-125a-3p. (G) Expression levels of miR-125a-3p in COAD and normal colon tissue, analyzed using UALCAN. (H) Relative expression of miR-125a-3p in COAD and normal colon cells, as measured by RT-qPCR. (I,J) Relative miR-125a-3p expression after transfection of SW620 cells was measured by RT-qPCR. (K,L) Relative TNK2 mRNA and protein expression were detected by RT-qPCR and western blotting after transfection. *p < 0.05, **p < 0.01. 显示更多 +