ab12031 ACK1 Antibody

Western blotting

Recommended dilution: 1:500-1:2000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500

 

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.

Description
Rabbit polyclonal antibody to ACK1

Applications 
WB, IF, ICC.

Immunogen 
ACK1 Antibody detects endogenous levels of total ACK1.

Reactivity 
Human,Mouse,Monkey.
可预测：Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)

Molecular weight
114kDa; 115kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ACK1

Synonyms 
Acetate kinase 1; Acetokinase 1; ACK 1; ACK; ACK-1; ACK1; ACK1_HUMAN; Activated Cdc42 associated kinase 1; Activated CDC42 kinase 1; Activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK 2; TNK2; Tyrosine kinase non receptor 2; Tyrosine kinase non receptor protein 2; Tyrosine kinase non-receptor protein 2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q07912

Western blot analysis of extracts from VERO cells, using ACK1 Antibody. The lane on the left was treated with blocking peptide. 显示更多 +

Western blot analysis of extracts from HepG2, using ACK1 Antibody. The lane on the left was treated with blocking peptide. 显示更多 +

ab12031 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab12031 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue). 显示更多 +