ab13160 Acid sphingomyelinase Antibody

Western blot analysis of extracts from HepG2 cells, using Acid sphingomyelinase Antibody. The lane on the left was treated with blocking peptide. Observed bands: 55 kDa.

ab13160 at 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab13160 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab13160 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

Fig. 2| mRNA and protein levels of acid sphingomyelinase(ASM) in diethylenetriamine/nitric oxide (DETA–NO)-treated HepG2 cells. a mRNA level of ASM in HepG2 cells treated with diferent concentrations of DETA–NO were assayed via real-time polymerase chain reaction. b Protein levels of ASM in HepG2 cells treated with diferent concentrations of DETA–NO were detected via western blotting. *P<0.05, **P<0.01,***P<0.001, compared with untreated HepG2 cells