ab10586 Acetyl-Histone H4 (Lys12) Antibody

Western blot analysis of extracts from various samples, Acetyl-Histone H4 (Lys12) Antibody. Lane 1: MCF7 treated with blocking peptide; Lane 2: MCF7 cells(TSA 1M, 18 hr); Lane 3: 3T3 cells(TSA 1M, 18 hr).

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Acetyl-Histone H4 (Lys12) Antibody. The lane on the left was treated with the synthesized peptide

ab10586 at 1/100 staining human Brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

ab10586 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF1021) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

ab10586 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab10586 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

Figure 5 (A-B) The level of histone deacetylase (HDAC) and histone acetyltransferase (HAT) in testis from mice treated for 30 days were measured by ELISA kits. Comparison between all groups was evaluated through One-way ANOVA. Mean±SD. n≥9. *p<0.05, compared with control group. ns, not significant. (C) The protein expression level of Histone H4 and acetylated H4 at different sites in testis. The representative photographs of proteins involved in Histone H4, acetyl-H4 (Lys5), acetyl-H4 (Lys8), and acetyl-H4 (Lys12) from Western Blots.