ab10584 Acetyl-Histone H3 (Lys23) Antibody

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500
 

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Rabbit polyclonal antibody to Acetyl-Histone H3 (Lys23)

Applications 
WB, IF, ICC.

Immunogen 
Acetyl-Histone H3 (Lys23) Antibody detects endogenous levels of Acetyl-Histone H3 only when acetylated at Lys23.

Reactivity 
Human, Mouse, Rat.
可预测：Bovine(100%)

Molecular weight
15 KD; 15kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
Acetyl-Histone H3 (Lys23)

Synonyms 
H3 histone family, member A;H3/A;H31_HUMAN;H3FA;Hist1h3a;HIST1H3B;HIST1H3C;HIST1H3D;HIST1H3E;HIST1H3F;HIST1H3G;HIST1H3H;HIST1H3I;HIST1H3J;histone 1, H3a;Histone cluster 1, H3a;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;H3.3A;HIST1 cluster, H3E;H3 histone family, member A;H3.1;H3/l;H3F3;H3FF;H3FJ;H3FL;Histone gene cluster 1, H3 histone family, member E;histone H3.1t;Histone H3/o;FLJ92264;H 3;H3;H3 histone family, member B;H3 histone family, member C;H3 histone family, member D;H3 histone family, member F;H3 histone family, member H;H3 histone family, member I;H3 histone family, member J;H3 histone family, member K;H3 histone family, member L;H3 histone family, member T;H3 histone, family 3A;H3/A;H3/b;H3/c;H3/d;h3/f;H3/h;H3/i;H3/j;H3/k;H3/t;H31_HUMAN;H3F1K;H3F3A;H3FA;H3FB;H3FC;H3FD;H3FH;H3FI;H3FK;HIST1 cluster, H3A;HIST1 cluster, H3B;HIST1 cluster, H3C;HIST1 cluster, H3D;HIST1 cluster, H3F;HIST1 cluster, H3G;HIST1 cluster, H3H;HIST1 cluster, H3I;HIST1 cluster, H3J;HIST1H3A;HIST1H3B;HIST1H3C;HIST1H3D;HIST1H3E;HIST1H3F;HIST1H3G;HIST1H3H;HIST1H3I;HIST1H3J;HIST3H3;Histone 1, H3a;Histone 1, H3b;Histone 1, H3c;Histone 1, H3d;Histone 1, H3e;Histone 1, H3f;Histone 1, H3g;Histone 1, H3h;Histone 1, H3i;Histone 3, H3;histone cluster 1 H3 family member a;histone cluster 1 H3 family member b;histone cluster 1 H3 family member c;histone cluster 1 H3 family member d;histone cluster 1 H3 family member e;histone cluster 1 H3 family member f;histone cluster 1 H3 family member g;histone cluster 1 H3 family member h;histone cluster 1 H3 family member i;histone cluster 1 H3 family member j;Histone cluster 1, H3a;Histone cluster 1, H3b;Histone cluster 1, H3c;Histone cluster 1, H3d;Histone cluster 1, H3e;Histone cluster 1, H3f;Histone cluster 1, H3g;Histone cluster 1, H3i;Histone cluster 1, H3j;Histone gene cluster 1, H3 histone family, member A;Histone gene cluster 1, H3 histone family, member B;Histone gene cluster 1, H3 histone family, member C;Histone gene cluster 1, H3 histone family, member D;Histone gene cluster 1, H3 histone family, member F;Histone gene cluster 1, H3 histone family, member G;Histone gene cluster 1, H3 histone family, member H;Histone gene cluster 1, H3 histone family, member I;Histone gene cluster 1, H3 histone family, member J;Histone gene cluster 1, H3A;Histone gene cluster 1, H3B;Histone gene cluster 1, H3C;Histone gene cluster 1, H3D;Histone gene cluster 1, H3E;Histone gene cluster 1, H3F;Histone gene cluster 1, H3G;Histone gene cluster 1, H3H;Histone gene cluster 1, H3I;Histone gene cluster 1, H3J;Histone H 3;Histone H3.1;Histone H3.2;Histone H3.3;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;Histone H3/m;H3 histone family 3A;H3 histone family 3B;H3 histone, family 3B (H3.3B);H3.3;H3.3A;H3.3B;H33_HUMAN;H3F3;H3F3A;H3f3b;Histone H3.3;Histone H3.3Q;Histone H3.A;Histone H3.B;MGC87782;MGC87783;

Storage
Store at -20°C. Stable for one year from the date of shipment. 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P68431 | Q71DI3 | P84243

Western blot analysis of extracts from EC304 cells(heat-shock treatment), using Acetyl-Histone H3 (Lys23) Antibody at 1/1000 dilution. Observed bands:15kD. 显示更多 +

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using Acetyl-Histone H3 (Lys23) Antibody at 1/1000 dilution. Observed bands:15kD. 显示更多 +

ab10584 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab10584 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue). 显示更多 +