ab10958 ACE2 Antibody

Western blot analysis of extracts from various samples, using ACE2 Antibody. Lane 1: PC12, treated with blocking peptide; Lane 2: PC12; Lane 3: Mouse heart; Lane 4: Mouse brain. Observed bands: 100 kDa.

Western blot analysis of ACE2 expression in Mouse brain lysate

ab10958 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab10958 staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab10958 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

FIGURE 3 Different distribution of angiotensin converting enzyme II (ACE2)‐expressing cells in normal and failed hearts. (a,b) Feature plot showing the distribution of ACE2, GJA4, and MYH6 expression levels in normal hearts (a) and in failed hearts (b). (c) Immunofluorescence staining of ACE2 (red) expression and DAPI, 4

Figure 1 Expression status of ACE2 in ccRCC tissues and cell lines. (A) Comparison of ACE2 mRNA expression in ccRCC and adjacent normal tissues (AN) based on GSE40435, GSE53757, GSE66272 and GSE126964 datasets. (B) Comparison of ACE2 protein expression in 54 pairs of matched paraffin section samples (ccRCC and adjacent normal tissue) using immunohistochemistry (C) Comparison of ACE2 protein expression in HK2 cell line and three ccRCC cell lines (786-O, OSRC2 and A498). ****p < 0.0001

Figure 7 ACE2 expression was associated with CD4 Naïve and CD4 Memory infiltration. (A) Correlation between ACE2 expression and CD4 Naïve, CD4 Memory and DC infiltration levels based TCGA and TIP data. (B) Correlation between ACE2 expression and CD4 and CD45 expression based on TCGA data. (C) Correlation between ACE2 protein expression and CD45RA and CD45RO protein expression based on the immunohistochemistry staining results of 54 clinical ccRCC samples.

further compare the transfection efficiency(Data are means ± SEM., n=2independent experiments. **P< 0.01 vs. ACE2@lipo 2000 (2 μg/mL)treated PMVECsin hypoxia).(C)Representative diagram of ACE2-CS-PRT stability within 7 days in PBS(Data are means ± SEM., n=3independent experiments).(D-E) Hemolysistest for assessment the hemolytic effect of ACE2-CS-PRT@PM by using rat blood red cell(Data are means ± SEM., n=3 independent experiments. **P< 0.01 vs. ACE2-CS-PRT 1mg/mL; ##P < 0.01 vs. ACE2-CS-PRT 2mg/mL).