ab12686 ABHD5 Antibody

Western blotting

Recommended dilution: 1:500-1:1000

 

Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfert of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion (By similarity).

Description
Rabbit polyclonal antibody to ABHD5

Applications 
WB, IHC

Immunogen 
ABHD5 Antibody detects endogenous levels of total ABHD5.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Molecular weight
39 kDa; 39kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ABHD5

Synonyms 
1-acylglycerol-3-phosphate O-acyltransferase ABHD5; ABHD5; ABHD5_HUMAN; Abhydrolase domain containing 5; Abhydrolase domain containing protein 5; Abhydrolase domain-containing protein 5; CDS; CGI 58; CGI58; CGI58 protein; IECN2; Lipid droplet-binding protein CGI-58; MGC8731; NCIE2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q8WTS1

ab12686 at 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. 显示更多 +

Figure 3 Immunohistochemical examination of CGI-58 in the (A) preeclampsia and (B) control (normal pregnant) groups. Samples were considered CGI-58-positive when brown-yellow granules were detected in the cytoplasm and cell membrane (red row). Magnification, x400. Scale bar, 50-µm. CGI-58, comparative gene identification-58. 显示更多 +

Figure 5 Representative expression of CGI-58 and LPL in control (normal pregnant, column 1, 2, 3) and preeclampsia (column 4, 5, 6) groups evaluated by western blotting. CGI-58, comparative gene identification-58; LPL, lipoprotein lipase. 显示更多 +