ab16730 ABCG8 Antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
ABCG8 |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:2000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
Immunohistochemistry
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.Description
Rabbit polyclonal antibody to ABCG8
Applications
WB, IF, ICC, IHC
Immunogen
ABCG8 Antibody detects endogenous levels of total ABCG8.
Reactivity
Human, Mouse, Rat.
可预测：Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%), Chicken(100%)
Molecular weight
76kDa; 76kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
ABCG8
Synonyms
ABCG8; ABCG8_HUMAN; ATP binding cassette sub family G (WHITE) member 8 (sterolin 2); ATP binding cassette sub family G member 8; ATP binding cassette, sub family G (WHITE), member 8; ATP-binding cassette sub-family G member 8; GBD4; MGC142217; Sterolin 2; Sterolin-2; STSL;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
Q9H221
产品图片
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Western blot analysis of extracts from Mouse testis, using ABCG8 Antibody at 1/1000 dilution. Observed bands:76kD. |
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ab16730 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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Figure 14 Immunohistochemical staining results. |
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FIGURE 5 Expression of the genes involved in cholesterol transport and bile-acid synthesis in the liver. (A) mRNA expression of Cyp7a1, Cyp8b1, Cyp7b1, and Cyp27a1 (n = 8). (B) Protein expression (left panel) and quantification (middle and right panel) of Cyp7a1 and Cyp7b1 (n = 6). (C) mRNA expression of Fgfr4 and β-klotho (n = 8). (D) mRNA expression of Abcg5 and Abcg8 (n = 8). (E) Protein expression (left panel) and quantification (middle and right panel) of Abcg5 and Abcg8 (n = 6). Data are the mean ± SD. *p < 0.05, **p < 0.01. Chow, chow diet; LD, lithogenic diet; AH, high-dose APS. |
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Figure 7 Hepatic expression of genes involved in cholesterol transport and synthesis. (A) Levels of expression of Abcg5 and Abcg8 mRNAs measured by qRT-PCR (left panel, n = 8) and RNA sequencing (RNA-seq) analyses (right panel, data shown as means). (B) Protein expression and quantification of Abcg5 and Abcg8 (n = 6). (C) Levels of expression of Lxrα, Hmgcr and Srebp2 mRNAs measured by qRT-PCR (left panel, n = 8) and RNA-seq analyses (right panel, data shown as means). Data are reported as means ± SEM. **P < 0.01. |
