ab16975 ABCC4 Antibody

Western blot analysis of extracts from COS-7, using ABCC4 Antibody. The lane on the left was treated with blocking peptide.
ab16975 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ab16975 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ab16975 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Figure 8. AP upregulates the relative mRNA and protein levels of FXR downstream bile acid transporters. (a–h) Relative mRNA levels of Mrp2, Mrp3, Mrp4, Oatp1, Ntcp, Bsep, Ost-α and Ost-β in the liver of mice by qPCR (n = 5 per group). (i–l) Relative protein levels of MRP2, MRP3, MRP4, OATP1 and NTCP in the liver of mice by western blot analysis (n = 3 per group). Data are presented as mean ± SEM. *p 

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

ABCC4

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:2000

 

Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

May be an organic anion pump relevant to cellular detoxification.

Description
Rabbit polyclonal antibody to ABCC4

Applications 
WB, IHC.

Immunogen 
ABCC4 Antibody detects endogenous levels of total ABCC4.

Reactivity 
Human, Mouse, Rat, Monkey.
可预测:Rabbit(80%)

Molecular weight
97kDa; 150kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ABCC4

Synonyms 
ABCC 4; ABCC4; ATP binding cassette sub family C (CFTR/MRP) member 4; ATP binding cassette sub family C member 4; ATP-binding cassette sub-family C member 4; bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4); bA464I2.1; Canalicular multispecific organic anion transporter; Canalicular multispecific organic anion transporter ABC superfamily; EST170205; MOAT B; MOAT-B; MOATB; MRP 4; MRP/cMOAT related ABC transporter; MRP/cMOAT-related ABC transporter; MRP4_HUMAN; Multi specific organic anion transporter B; Multi-specific organic anion transporter B; Multidrug resistance associated protein 4; Multidrug resistance-associated protein 4; OTTHUMP00000018560;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
O15439

产品图片

Western blot analysis of extracts from COS-7, using ABCC4 Antibody. The lane on the left was treated with blocking peptide.

ab16975 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab16975 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab16975 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Figure 8. AP upregulates the relative mRNA and protein levels of FXR downstream bile acid transporters. (a–h) Relative mRNA levels of Mrp2, Mrp3, Mrp4, Oatp1, Ntcp, Bsep, Ost-α and Ost-β in the liver of mice by qPCR (n = 5 per group). (i–l) Relative protein levels of MRP2, MRP3, MRP4, OATP1 and NTCP in the liver of mice by western blot analysis (n = 3 per group). Data are presented as mean ± SEM. *p 

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