ab10453 5HT5A receptor Antibody

Western blot analysis of extracts from various samples, using 5-HT-5A Antibody. Lane 1: Mouse brain lysates; Lane 2: Rat brain lysates; Lane 3: Rat brain lysates treated with blocking peptide;
ab10453 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ab10453 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

5HT5A receptor

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution: 1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.


Description

Rabbit polyclonal antibody to 5HT5A receptor


Applications 
WB, IF, ICC, IHC

Immunogen 
5HT5A receptor Antibody detects endogenous levels of total 5HT5A receptor.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Molecular weight
Horse(80%), Rabbit(90%)

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
5HT5A receptor

Synonyms 
HTR5A; 5 HT 5A; 5 hydroxytryptamine 5A receptor; 5-HT-5; 5-HT-5A; 5-HT5A; 5-hydroxytryptamine receptor 5A; 5HT5A; 5HT5A_HUMAN; HTR5A; Serotonin receptor 5A;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P47898
 


产品图片

Western blot analysis of extracts from various samples, using 5-HT-5A Antibody. Lane 1: Mouse brain lysates; Lane 2: Rat brain lysates; Lane 3: Rat brain lysates treated with blocking peptide;

ab10453 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

ab10453 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

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