ab13652 5HT1D Receptor Antibody

Western blotting

Recommended dilution: 1:500-1:2000
 

Immunohistochemistry

Recommended dilution: 1:50-1:200

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.

Description
Rabbit polyclonal antibody to 5HT1D Receptor

Applications 
WB, IHC

Immunogen 
5HT1D Receptor Antibody detects endogenous levels of total 5HT1D Receptor.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(89%)

Molecular weight
45 kDa; 42kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
5HT1D Receptor 

Synonyms 
5 HT 1D alpha; 5 HT 1D; 5 HT1B; 5 HT1D; 5 hydroxytryptamine (serotonin) receptor 1B; 5 hydroxytryptamine (serotonin) receptor 1D G protein coupled; 5 hydroxytryptamine 1B receptor (5 HT 1B) (Serotonin receptor) (5 HT 1D beta) (Serotonin 1D beta receptor) (S12); 5 hydroxytryptamine receptor 1D; HT1DA; HTR1D; HTR1D2; HTR1DA; HTRL; RDC4; S12; Serotonin receptor 1D;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P28221

ab13652 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody. 显示更多 +

Fig. 2. Effect of 5-hydroxytryptamine receptor 1D (HTR1D) knockdown in gastric cancer (GC) cells on HTR1D function in vitro and in vivo. Effect of HTR1D knockdown on (A) proliferation, (B) clonogenic ability, and (C) migration of AGS and MGC803 cells. Magnification of migration experiment was 200 × . (D) Subcutaneous tumor nodules were generated by subcutaneously injecting short hairpin NC (shNC) and shHTR1D-1 MGC803 cells into nude mice. (E) Tumor growth curves in shNC and shHTR1D-1 groups. (F) Weight of harvested subcutaneous tumors in shNC and shHTR1D-1 groups. (G) Immunohistochemical staining of HTR1D and Ki67 in subcutaneous tumors in shNC and shHTR1D-1 groups. 显示更多 +

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