ab11223 5HT1A Receptor Antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
5HT1A Receptor |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:2000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
Immunohistochemistry
Immunohistochemistry-Paraffin
Recommended dilution: 1:50-1:200
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.Description
Rabbit polyclonal antibody to 5HT1A Receptor
Applications
WB, IF, ICC, IHC, IHC-p
Immunogen
5HT1A Receptor Antibody detects endogenous levels of total 5HT1A Receptor.
Reactivity
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Molecular weight
46 kDa; 46kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
5HT1A Receptor
Synonyms
5 HT 1A; 5 HT receptor 1A; 5 HT1A; 5 HT1A receptor; 5 hydroxytryptamine (serotonin) receptor 1A; 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled; 5 hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; 5-hydroxytryptamine receptor 1A; 5HT 1A; 5HT 1A receptor; 5HT receptor 1A; 5HT1a; 5HT1A_HUMAN; ADRB2RL1; ADRBRL1; G 21; G protein coupled receptor; G-21; Gpcr 18; Gpcr18; Guanine nucleotide binding regulatory protein-coupled receptor; Htr1a; PFMCD; Serotonin 1A receptor; Serotonin 5HT1A receptor; Serotonin receptor 1A;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
P08908
产品图片
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Western blot analysis of extracts from 293, using 5-HT-1A Antibody. The lane on the left was treated with blocking peptide. |
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Western blot analysis of extracts from various samples, using 5HT1A Receptor Antibody. Lane 1: Hepg2 cells(serum starvation treatment), blocked with antigen-specific peptides. Lane 2: Hepg2 cells(serum starvation treatment). Lane 3: Hela cells(heat-shock treatment). |
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ab11223 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. |
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ab11223 staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab11223 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue). |
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Figure 3 Comparison of the expression levels of HTR1As and NR2B in bilateral insular cortexes among three groups. The mRNA and protein expression levels of HTR1As (A and C) and NR2B (B and D) in bilateral insular cortexes were compared among WAS, sham WAS and normal control groups. (E) and (F): Representative immunoblots from the bilateral insular cortexes show the expression of NR2B, 5HTR1A and GAPDH, with an indication of size (kilodaltons, kDa). *represented that the difference was significant, p < 0.05. WAS, water avoidance stress. |
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Figure 3. Effects of CREOs on 5HT-1A and GR expressions in cortex, hippocampus, and hypothalamus. (A) Immunohistochemistry images of 5HT-1A (brown), scale bar = 50-μm. (B) Quantification analysis of 5HT-1A positive cells. (C) Immunohistochemistry images of GR (brown), scale bar = 50-μm. (D) Quantification analysis of GR-positive cells. Data were expressed as the Mean ± SD (n = 6). The significance of differences versus the Res group is at *P |
