ab11606 4E-BP1 Antibody
品牌 |
|
|---|---|
产品货号 |
|
来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
4E-BP1 |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution: 1:500-1:2000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
Immunohistochemistry
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.Description
Rabbit polyclonal antibody to 4E-BP1
Applications
WB, IF, ICC, IHC
Immunogen
4E-BP1 Antibody detects endogenous levels of total 4E-BP1..
Reactivity
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Molecular weight
18kDa; 13kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
4E-BP1
Synonyms
4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
Q13541
产品图片
|
|
Western blot analysis of extracts from Myeloma cells, using 4E-BP1 Antibody. Lane 1 was treated with the blocking peptide. |
|
|
Figure 8. (Fan). |Protein amounts of mTOR pathway efectors. (A) Representative immunoblot bands of various proteins. |
|
|
Fig. 8. |Western blot analysis of TOR protein phosphorylation (p-TOR Ser2448) (A), S6K1 protein phosphorylation (p-S6K1 Thr-389) (B), 4EBP1 protein phosphorylation (Thr-37/46) (C) in the gill of on-growing grass carp fed diets containing graded levels of Br-DMPT for 60 days, then infected with F. columnare for 3 days.Data represent means of six fish in each group, error bars indicate S.D. Values having different letters are significantly different (P < 0.05). |
