ab14354 14-3-3 theta Antibody

Western blot analysis of extracts from various samples, using 14-3-3 theta Antibody. Lane 1: Raji cells, blocked with antigen-specific peptides, Lane 2: Raji cells, Lane 3: COLO205 cells, Lane 4: Rat spleen, Lane 5: HepG2 cells.
ab14354 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.

品牌

产品货号

来源种属

Rabbit

抗体克隆

Polyclonal

来源亚型

IgG

实验方法

WB,IHC,IF,ICC

实验种属

Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep

偶联标记

Unconjugated

目的蛋白

14-3-3 theta

产品规格

50μl,100μl,200μl

产品报价

¥1500/¥2750/¥3600

实验应用

Western blotting

Recommended dilution:1:500-1:1000


Immunofluorescence

Recommended dilution: 1:100-1:500


immunocytochemistry

Recommended dilution: 1:100-1:500


Immunohistochemistry

Recommended dilution: 1:50-1:200

 



最佳稀释倍数与浓度应由实验研究人员确认

产品说明



产品背景

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Description
Rabbit polyclonal antibody to 14-3-3 theta

Applications 
WB, IF, ICC, IHC

Immunogen 
14-3-3 theta Antibody detects endogenous levels of total 14-3-3 theta.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)

Molecular weight
28 KD; 28kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
14-3-3 theta 

Synonyms 
14 3 3 protein T cell; 14 3 3 protein tau; 14 3 3 protein theta; 14 3 3 tau; 14 3 3 theta; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 1433T_HUMAN; 1C5; HS1; KCIP1; Protein HS1; Protein kinase C inhibitor protein 1; Protein tau; tyr3/trp5 monooxygenase activation protein, theta; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide; Ywhaq;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P27348



 

产品图片

Western blot analysis of extracts from various samples, using 14-3-3 theta Antibody. Lane 1: Raji cells, blocked with antigen-specific peptides, Lane 2: Raji cells, Lane 3: COLO205 cells, Lane 4: Rat spleen, Lane 5: HepG2 cells.

ab14354 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.

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