ab14352 14-3-3 eta Antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IF,ICC |
实验种属 |
Human,Mouse,Rat,Rabbit,Pig,Dog,Chicken,Bovine,Horse,Sheep |
偶联标记 |
Unconjugated |
目的蛋白 |
14-3-3 eta |
产品规格 |
50μl,100μl,200μl |
产品报价 |
¥1500/¥2750/¥3600 |
实验应用
Western blotting
Recommended dilution:1:500-1:1000
Immunofluorescence
Recommended dilution: 1:100-1:500
immunocytochemistry
Recommended dilution: 1:100-1:500
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.Description
Rabbit polyclonal antibody to 14-3-3 eta
Applications
WB, IF, ICC
Immunogen
14-3-3 eta Antibody detects endogenous levels of total 14-3-3 eta.
Reactivity
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)
Molecular weight
42 KD; 28kD(Calculated).
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Full name
14-3-3 eta
Synonyms
14 3 3 protein eta; 14-3-3 eta; 14-3-3 protein eta; 1433F_HUMAN; Brain protein 14-3-3, eta isoform; HGNC:12853; Protein AS1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1; YWHA1; Ywhah;
Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Swissprot
Q04917
产品图片
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Western blot analysis of extracts from Rat testis, using 14-3-3 eta Antibody. The lane on the left was treated with blocking peptide. |
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ab14352 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14352) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue). |
