ab14350 14-3-3 beta Antibody

Western blotting

Recommended dilution:1:500-1:1000

 

Immunofluorescence

Recommended dilution: 1:100-1:500

 

immunocytochemistry

Recommended dilution: 1:100-1:500

 

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

Description
Rabbit polyclonal antibody to 14-3-3 beta

Applications 
WB, IF, ICC

Immunogen 
14-3-3 beta Antibody detects endogenous levels of total 14-3-3 beta.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Molecular weight
28 KD; 28kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
14-3-3 beta

Synonyms 
14 3 3 alpha;14 3 3 protein beta/alpha;14-3-3 protein beta/alpha;1433B_HUMAN;Brain protein 14 3 3 beta isoform;GW128;HS 1;KCIP-1;KCIP1;N-terminally processed;Protein 1054;Protein kinase C inhibitor protein 1;YWHAA;YWHAB.

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P31946

ab14350 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14350) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue). 显示更多 +

Western blot analysis of extracts from various samples, using 14-3-3 beta Antibody. Lane 1: Raji cells, blocked with antigen-specific peptides, Lane 2: Raji cells, Lane 3: COLO205 cells, Lane 4: HepG2 cells. 显示更多 +