ab100020 AFP antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,ELISA |
实验种属 |
Human |
偶联标记 |
Unconjugated |
目的蛋白 |
AFP |
产品规格 |
25μl,100μl,200μl |
产品报价 |
¥1080/¥2600/¥3600 |
实验应用
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 50-200
Predicted cell location: Cytoplasm
Positive control: Human prostate cancer
Recommended dilution: 50-200
Western blotting
Predicted band size: 69 kDa
Positive control: Human liver cancer tissue lysate
Recommended dilution: 1000-5000
ELISA
Recommended dilution: 2000-10000
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
产品背景
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in inpiduals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Storage
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Description
Anti-AFP rabbit polyclonal antibody
Applications
ELISA, WB, IHC
Immunogen
Fusion protein of human AFP
Reactivity
Human
Content
0.5 mg/ml
Host species
Rabbit
Ig class
Immunogen-specific rabbit IgG
Purification
Antigen affinity purification
Symbol
AFP
Full name
alpha fetoprotein
Synonyms
AFPD; FETA; HPAFP
Swissprot
P02771
产品图片
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The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ab100020(AFP Antibody) at dilution 1/50. (Original magnification: ×200) |
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The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using ab100020(AFP Antibody) at dilution 1/50. (Original magnification: ×200) |
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human liver cancer tissue lysate, Primary antibody: ab100020(AFP Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds |
