ab100008 c-Myc Tag antibody
品牌 |
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产品货号 |
|
来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,ELISA |
实验种属 |
Human,Mouse,Rat,Rabbit,Cow,Pig,Dog |
偶联标记 |
Unconjugated |
目的蛋白 |
Myc-Tag |
产品规格 |
25μl,100μl,200μl |
产品报价 |
¥700/¥1600/¥2600 |
实验应用
Western blotting
Predicted band size:50 kDa
Positive control:Fusion proteins containing the c-Myc Tag
Recommended dilution: 2000-10000
ELISA
Recommended dilution: 5000-10000
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
产品背景
A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
保存缓冲液
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
运输条件
2-8°C
靶点 Uniprot 登记号
P01106
靶点基因 ID
4609
靶点全称
Myelocytomatosis oncogene Tag
靶点别名
c- Myc epitope Tag
靶点研究领域
Epigenetics and Nuclear Signaling, Cancer, Signal Transduction
亚型
Immunogen-specific rabbit IgG